The present study was undertaken to investigate the antidiabetic effect of the bitter melon and black cumin on alloxan induced diabetes in experimental rabbits. At 2 to 3 months of age, rabbits were assigned into five groups (A, B, C, D and E) and rabbits were included each group. Group A was kept as control, Group B was treated with alloxan intramuscularly at a dose of 75mg /kg body weight, Group C was treated with bitter melon at a dose of 150gm/kg body weight orally, Group D was treated with black cumin at a dose of 250mg/kg body weight orally, Group E was treated with combined at previous dose. After acclimatization, diabetes was induced in four groups of rabbits (B, C, D and E) by administering alloxan injection. There was significant (p<0.05) decreased in blood glucose level in all treated group C, D, E compared to the B group and lowest glucose was recorded in E group when treated with combined medicinal herbs and body weight was increased in all treated group C, D, E compared to the B group and highest was recorded in D group while treated with those. % of PCV level and Hbgm/dl concentration was the highest in group E which was treated with both black cumin and bitter melon compare to the A group. ESR was highest in group B treated with alloxan and lowest in group E. The present study demonstrated that combined treatment increased body weight and decreased glucose level without affecting health of rabbits.