Author Archives: aajbb

The present study was designed with a view to identify the Avian Leukosis Virus (ALV) by direct Enzyme Linked Immunosorbent Assay (ELISA) as well as to know the prevalence of ALV in different age groups of layer birds at Dinajpur district of Bangladesh. The birds were categorized into three groups, namely group A (brooder) included bird aged 13 days, group B (grower) included bird aged 16 weeks and lastly group C (layer) included bird aged 32 weeks. In this study a total of 92 cloacal swab samples were examined and 13 positive cases of ALV was found among which 5, 4 and 4 positive samples were in grower, brooding and layer chickens respectively. This study showed that ALV positive cases were 14.13% in layer birds whereas 14.5% positive cases in Sonali chicken and 13.3% in case of Hy-Line brown chicken. The inoculation of cloacal swab was done into five 10 days old embryonated chicken eggs through yolk sac route. After 5 days of inoculation death of the all embryos with haemorrhage was observed indicating the prevalence of Avian Leukosis Virus in the study area of Bangladesh. Since Avian Leukosis Virus transmitted both horizontally and vertically therefore measures to prevent spread are more demanding.

Anemia in human being due to iron deficiency would be solved with the intake of iron rich rice (Oryza sativa L.) grain developed by biofortification. Phenotypical study of obsolete rice germplasms containing high iron content is important. Phenotypic characters like flowering and maturity time; plant height; tillers hill^‑1; grains panicle^‑1; seed weight and grain yield plant^‑1 were studied for 39 rice genotypes in this research. In this research, Malagoti showed early maturity (126 day), Dhar Shail showed highest  filled grain panicle^-1 (303), Marish Shail showed highest grain yield plant^-1 (86.66g),  and Karengal showed lowest  filled grain panicle^-1 (59.67) and lowest  grain yield plant^-1 (17.20g). Number of filled grains panicle^-1 showed high heritability. All the yield contributing traits except panicle length and unfilled grains panicle^-1 were significantly and positively correlated with grain yield plant^-1 at both phenotypic and genotypic level. The highest direct effects (0.486 and 9.75) on grain yield plant^-1 were found for plant height and days to maturity at phenotypic and genotypic level with residual effect 0.461 and 0.496 respectively. Finally, the obsolete genotypes with highest grain yield (Marish Shail) and early maturity (Malagoti)  performance would be used as breeding materials to improve iron rich rice varieties.

Iron deficiency causes anemia in human body. So identification of iron rich rice genotypes as well as biofortification of staple food crops is an effective way to overcome such malnutrition problems. A total of 46 local rice landraces of Bangladesh were used in chemical analysis and DNA fingerprinting to study their ability to synthesize and accumulate iron content in the grain. Rice plants were grown and their grains were collected and digested by acidKNO₃:HClO₄. iron concentrations were measured from The highest iron content was found Kumra Ghor (168.52ppm) and the lowest in Patnai Balam (0.45 ppm). The SSR markers were used to determine the allelic diversity and relationship among the selected rice germplasms for iron content. Out of 10 SSR primers, 5 primers showed DNA amplification and polymorphism among all the genotypes. Variation was found in number of alleles, allele frequency, Polymorphism Information Content (PIC) and gene diversity. The primer, RM35 having motif (GA) 19 also yielded the highest number of alleles (23) and highest PIC value (0.946). A total of 72 bands were recorded by using 5 SSR primers in all genotypes. The number of alleles per locus ranged from 4 to 23 with an average of 14.40 out of 72 bands. The UPGMA Dendrogram based on Nei’s (1973) genetic distance placed the varieties into 6 distinct clusters. Most of the primers showed the highest Polymorphism Information Content (PIC). Based on this study, the larger range of similarity values using SSR markers provides greater confidence for the assessment of genetic relationships among the varieties. The information obtained from chemical analysis and SSR profiling helps to identify the varieties containing iron content. Genotypes with high iron content could be used as breeding materials to develop nutrient rich rice varieties in order to combat iron deficient problems in population of Bangladesh.