To evaluate the effect of nutrients enriched feed premix on the growth performance of major carps Rohu (Labeo rohita), Catla (Catla catla) and Mrigal (Cirrhinus mrigala) in a polyculture system, the present experiment was carried out in six earthen ponds located at Khulna University of Bangladesh for a period of 90 days. Two treatments each with three replications were stocked with 40% Rohu, 30% Catla and 30% Mrigal with a total stocking density of 10000/ha. T-2 was treated with feed premix at the rate of 4g/1 kg feed while T-1 was not treated with any feed premix. Both treatments were applied normal feed twice a day at the rate of 2% to 4% of the body weight of the fingerlings. The net fish production was found to be 2238.06 kg/ha and 3607.33 kg/ha in T-1 and T-2 respectively. The results showed that fish production was significantly higher in T-2 than T-1 (P<0.05). T-2 showed 0.60 times greater fish production than that of T-1.