This study was conducted to know prevalence of gestational diabetes and associated factors. A cross sectional data was collected from the pregnant mothers from a hospital of Barisal city, Bangladesh. It was revealed that 5% of the interviewed mothers suffer from diabetes. Among 100 participants, 58% were in 20-30 years age group and 68% patients married within 21-25 years old. Near about half (51%) of mothers had more than 60 Kg body weight. Maximum (48%) patients passed their secondary education and 78% were jobless. More than half (56%) of the patient’s family monthly earned 10000-20000 BD Tk. Among the mothers, 15% had the family history of diabetes. Correlation matrix revealed that, body weight, and blood pressure were positively associated with sugar level. Gestational age (P=0.04261) and diastolic blood pressure (P=0.018764) were significantly associated with the RBS of pregnant mothers. More study should be conducted to explore the accurate prevalence and practices of diabetic patients in the study area.