Infection control and standard precautions is evidence-based practices that can reduce the risk of transmission of microorganisms. Infection control education is a fundamental component of the Nursing curriculum. A cross-sectional study was conducted to assess the level of knowledge and practices regarding infection control among nurses in secondary level hospital at 250 beds Chittagong General Hospital and Fouzderhat TB Hospital, Chittagong. The study period was 1^st January to 31^st December 2018. Total 182 nurses were included in this study. The respondents were selected by purposive sampling technique and data were collected by face to face interview. The study was found that (78.0%) respondents had fair knowledge and (90.2%) had fair practices regarding infection control. The study also revealed that, 161 (88.5%) respondents awarded about hand washing, 179 (98.4%) respondents were concerned about waste management and 93 (51.1%) respondents agree and 85 (46.7%) respondents strongly agree about PPE use is important. Significant statistical difference was found between working experience and knowledge level. This study can be concluded that in spite of practice level regarding infection control, nurses had fair knowledge level. These finding suggest that nurse knowledge and practice regarding infection control need further improvement.