In order to know the present status of aqua-medicines used in aquaculture activities in Jessore district, Bangladesh a rigorous investigation was conducted with fish farmers and representative of pharmaceutical companies from January, 2016 to May 2016. An extensive assortment of aqua-medicines was comprehended throughout pond prepation and water excellence administration by the agrarians. Amongst them Zeo-Fesh, Zeo Prime, Ecolite, Megazeo plus, Geotox, Jv Zeolite, Acmes, Benthos, Pond life, were extensively used in field level. Disinfectants has precise significance; far and wide used by the agrarians and the furthermost prevalent disinfectants are Pathonil, Timen, Fmsen, Virex, Aquakleen etc., the reimbursements of disinfectants are to inhibit dissimilar categories of bacterial, fungal and viral diseases resembling gill rot, tail rot, dropsy etc. to uphold sanitized circumstance in pond apparatus and floorings and in some circumstances use to treat disease. Numerous aqua-medicines were established in the aqua-medicines retailers that were informed to be used as noxious gas reducer. The obtainable deadly gas reducer were gastrap, gas stop, gasonex plus, ammonil besides that, remunerations of using venomous gas reducer are to eliminate the lethal gas like NH₃, H₂S, CO₂ etc. since the bottommost of pond to diminish concentration of ammonia. For upgrading of dissolve oxygen level, Oxyflow, Oxymax, Bio Care, Bio-Ox, Oxy-Gold, Oxy-A, Oxylife were extensively used in the study area. A number of aqua medicines were found in the aqua-medicine enterprise used as growth promoter in addition to upsurge yield performance like, Megavit Aqua, Aqua Boots, Aquamin, Acimix etc. were extensively used in study area. Countless pharmaceutical companies have been chronicled to marketplace these aquaculture healthcare products. Furthermost of the products have been promoted from diverse republics like USA, Thailand, Malaysia, Belgium, and China and values were inconstant, but appeared to be reasonable to agrarians. The contemporaneous investigation pointed out numerous restraints concomitant with the use of such aqua-medicines, comprising nonexistence of methodological acquaintance of agrarians about use of aqua-medicines.