2016

Sleep disordered breathing is a spectrum of diseases that includes snoring, upperairway resistance syndrome and obstructive sleep apnea. It occurs more in obese males, resulting in hypertension and cardiac complications if untreated. There are various treatments, conservatively and surgically, starting from simple tonsillectomy to multi-level surgeries and multi discipline. Literature review was carried out on the pathophysiology, clinical presentation, complications, diagnosis and various treatments, using internet Google, search PubMed. Additional information was obtained by cross referencing, using text and journals in the medical libraries.

Malathion is an organophosphorous insecticide widely used in the agricultural field in Bangladesh and reach in the aquatic environment through rain wash. In the present study, we examined the effects of Malathion on hematological parameters and gills morphology in common carp exposed to two sub-lethal concentrations (1.5 and 3.0 mg/L) for a period of 192h (8 days). For hematological parameters fish were sacrificed at 24, 48, 72, and 96h after start of exposure. Gills were collected at 192h after start of exposure. The blood glucose level was significantly elevated with increasing the concentration of Malathion. Red blood cells (RBCs), hemoglobin (Hb) and hematocrit (Hct) values were significantly decreased after the exposure of Malathion. Significant increments were observed in case of the mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), while no distinct changes were noted for mean corpuscular hemoglobin (MCH). Interestingly, white blood cells (WBCs) count was significantly increased with toxicity of Malathion. Several morphological changes, such as telangiectasia, blood lamellar congestion, hypertrophy of filaments, lamellar fusion were observed in the gills of fish exposed to Malathion. The present study revealed that the insecticide had adverse effects on various blood parameters and gills morphology in common carp. Thus, the use of insecticide in the agriculture field may be a threat to fauna and flora of the aquatic environment.

The rheumatoid arthritis (RA) is an auto-immune, rheumatic and chronic inflammatory disease, characterized by joints damage.  The early diagnosis of RA allows the initiation of a treatment which offers to the patients more chance of remission and avoids the evolution towards the unrecoverable deformity of joints.  The objective of this study is to evaluate the performance of recent tests for the determination of anti -CCP antibodies and FR by ELISA in Benin Republic. This analytical, retrospective (2 years 6 months) and prospective (7 months) study allowed us to collect 36 patients meeting the American College of Rheumatology (ACR) criteria for RA and 24 controls. A comparison was made with the latex agglutination test for rheumatoid factors and a search of rheumatoid factors (RF) on the one hand and anti-cyclic citrullinated peptide. In our study, the specificity of anti-CCP assay (100 %) is higher than that of RF-ELISA (91.7%).  The sensitivity of RF-ELISA assay is higher (77.8 %) than that of anti-CCP assay (66.7%). The latex test for rheumatoid factors has a sensitivity of 33.3 %. The positive predictive value (PPV) of anti-CCP assay (100 %) is higher than that of RF-ELISA assay (93.33 %).  The positive-likelihood ratio (LR+) of anti-CCP assay is higher than the LR+ of RF-ELISA assay (4.96).  The negative-likelihood ratio (LR-) of anti-CCP assay (0.33) is higher than the LR- of RF-ELISA assay (0.24).  In conclusion, the anti-CCP assay has the highest specificity and RF-ELISA assay shows the highest sensitivity.  In conclusion, the association of the two assays enhances a better diagnosis value for RA.

Tobacco smoking in adolescent age group has become a major public health concern as it is one of the major risk factors for non-communicable diseases leading to burden of adverse health effects in their later life. Those who start smoking tobacco at an early age are more likely to develop nicotine addiction and continue smoking throughout their adulthood. The general objective of the study was to determine the prevalence of tobacco smoking, their initiation and perception towards tobacco smoking among school adolescents in Kudat District, Sabah State, Malaysia. It was a school based cross sectional descriptive study which employed a twostaged stratified random sampling method and used self-administered structured questionnaire for data collection. A total of 257 students aged between 15 to 19 years old from two upper secondary schools participated in the study. This study revealed that (26.6%) were ever smokers and (8.6%) were current smokers. The earliest age of started smoking was 7 years old and 9 years old among male and female ever smokers respectively. Majority of the respondents perceived that smoking was a bad habit which would cause environmental pollution and harmful effects on health. The most common reasons to start smoking among ever smokers were peer pressures, curiosity and experimenting and to relieve the feeling of pressured and stress. Among the current smokers, 86.4%had ever attempted to quit smoking but they failed to stop smoking because they thought smoking could give feeling pleasure (50%), relieve their anxiety or craving (27.3%) and because of peer pressures (22.7%). The findings suggested that although tobacco smoking prevalence among school adolescents in Kudat was comparatively lower than that of national prevalence reported by GYTS, there is still need for early interventions with holistic approach to prevent them from initiation and to help them for cessation of tobacco smoking.

The extensive progress of dairy sectors in a developing country like Bangladesh, led to widespread use of antibiotics to improve the health and productivity of animals. Prolonged usage may lead to antibiotic residues in foods of animal origin; hence, the emergence of antimicrobial resistant microorganisms. Accurate data on the antibiotic usage in livestock treatment, antibiotic residues and antimicrobial resistances in raw milk in Bangladesh are lacking. This study aimed to investigate the types and usages of antibiotics in cattle, their potential microbial resistances in raw milk samples. To do so, a total of 54 raw milk samples were evaluated and the bacterial isolates were identified and measured for resistance to 4 antibiotics most commonly used during bacterial infection Bangladesh. Amongst all 54 (100%) isolates were positive to S. aureus and 36 (66.67%) isolates were positive to E coli. Determination of the antibiotic resistance pattern of isolates showed that all isolates of S. aureus were resistant to cefoxitin (81.48%), ampicillin (64.81%), ciprofloxacin (51.85%), and gentamycin (70.37%). E. coli showed resistance to cefoxitin (69.44%), ampicillin (83.33%), ciprofloxacin (77.78%), and gentamycin (86.11%). However multidrug resistance pattern was also found. The obtained results provide evidence that antimicrobial resistant strains of the above pathogens have become remarkably widespread in raw milk. This requires better management for antibiotic usages among livestock farmers to control sources of food contamination and reduce the health risks associated with the development of resistant microbial strains.

The present study was undertaken to investigate the antidiabetic effect of the Bitter melon and Garlic on Alloxan induced diabetes in experimental rabbits. At 2 to 3 months of age, rabbits were assigned into five groups (A, B, C, D and E) and each group was remained 4 rabbits. Group A was kept for control, Group B was treated with Alloxanintramuscullarly at a dose of 75mg /kg body weight, Group C was treated with bitter melon 250gm/kg body weight orally, Group D was treated with garlic 750mg/kg body weight orally, Group E treated with combined at previous dose. After acclimatization, diabetes was induced in four groups of rabbits (B, C, D and E) by administering Alloxan injection in a dose of 75mg/kg body weight (b.wt.) intramuscularlly. There was significant decreased in blood glucose level in all treated group C, D, E compared to the B group and lowest glucose was recorded in E group when treated with combined medicinal herbs and body weight  was increased in all treated group C, D, E compared to the B group and highest was recorded in Dgroup while treated with those.% of PCV level and Hb gm/dl concentration was the highest in group E which was treated with both garlic and bitter melon compare to the A group. ESR was highest in group B treated with Alloxan and lowest in group E. The present study reveals that combined treatment increases body weight and decreases glucose level without affecting health of rabbits.

This study was conducted to determine the efficacy of anthelmintics against gastrointestinal parasite in periparturient dairy cows and its effect on milk yield and quality. Sixty mixed breed pregnant cows were selected divided into four groups, A (n=18), B (n=14), C (n=16) and D (n=12) and group D kept as control. Cows of group A were treated with Nitroxynilat the rate of 10 mg/kg bwt. s/c, 30 days before parturition and average feacal egg per gram(EPG) was reduced from 506.1 ± 172.3 to 157.8 ± 24.9 within 14 days.Group B were treated with combined triclabendazole and levamisoleat the rate of19.5 mg/kg bwt. orally at calving. The average EPG was reduced from 967.7 ± 237.1 to 172.0 ± 25.9. Group C were treated with same as group B at calving and 42 days after. The mean EPG fall from 794.6 ± 310.5 to 166.7 ± 26.2.In group D average EPG increased from 791.7 ± 268.5 to 864.2 ± 290.7.  The mean change in EPG 14 days after treatment was significantly higher (p<0.05) in treated (79.1%) cows than control (-9.2%). The average milk yield in all treated cows was significantly (p<0.01) higher in treated lactation (2.5 ± 0.7) than previous lactation (2.2 ± 0.7) and control cows (2.2 ± 0.8). Although, milk yields were higher in second parity (2.5 ± 0.7) than the first (2.4 ± 0.7), there was no significant difference (p>0.05). The percentage of milk fat, protein, lactose, SNF was significantly higher (P<0.05) in treated group than the control group.

The study was conducted at Ma-Fatema Fish Hatchery in Jessore from January to March, 2016. The present study was emphasized on water quality parameters, feeding management and cost- benefit analysis. The water quality parameters temperature (⁰C), pH, dissolved oxygen (DO) (mg/l) and transparency (cm) were measured by Celsius thermometer, pH meter, DO meter and secchi disk, respectively. The proximate composition of feed ingredients was determined by the Association of Analytical Chemists, 1980. Brood fishes were reared all year round in the brood ponds by supplying formulated feeds. Temperature was 19^oC -26°C in brood ponds and 18^oC-24^oC in hatching jars; pH was 6.93–7.87 in brood ponds and 6.79-7.54 in the hatching jars; dissolved oxygen was 4.8-6.3 mg/l in brood ponds and 4.8-6.7 mg/l in hatching jars; transparency was 14-18 cm in the brood ponds. The percentage of farm made feed was protein (27.76%), lipid (4.9%), CHO (18.62%), ash (11.31%), fiber (6.4%), moisture (31.01%). The cost benefit ratios were 1.54, 1.32 and 1.23 and net profits were BDT 49911.67, BDT 24816.67and BDT 16366.67 respectively in January, February and March. Management of brood fish by providing quality feed and physico-chemical parameters of water were maintained in according to scientific procedure. Cost-benefit ratio of the hatchery in common carp production was satisfactory.

The present study was undertaken for the detection of arsenic in different organs or tissues in an experimentally induced arsenicosis affected adult Newzealand white rabbits. The experiment was carried out on a total of 30 (01 month old) adult Newzealand white rabbits. Experimental arsenicosis were developed in rabbits by oral administration of arsenic trioxide alone and along with tannic acid, di-sodium hydrogen phosphate (DSHP), alum and As contaminated water after filtration using SCIF bed along with alum.   For this, the rabbits were randomly divided in to six (6) equal groups (A,B,C,D,E & F) at the ratio of three males and two females in each group, rats of group A was kept as control without giving any treatment, rabbits of group B received arsenic trioxide@100ppm, group C received arsenic trioxide @ 100ppm plus tannic acid @100ppm, group D received arsenic trioxide @ 100ppm plus di-sodium hydrogen phosphate (DSHP) @100ppm, group E received arsenic trioxide @100ppm plus alum @100ppm orally daily for 60 days in all cases and group F received alum@100ppm in SCIF-bed filtrated water  orally daily for 60 days. The different organs and tissues of both dead and sacrificed rabbits (Liver, kidney, heart, Lungs spleen, stomach, muscle and skin) were collected for detection of arsenic. Arsenic was detected qualitatively by Reinsch test, semiquantitatively by Merck Arsen test kit. The distribution of arsenic concentration was highest in liver and lowest in skin. It has been concluded that arsenic were deposited in different organs or tissues of rabbit in an experimentally induced arsenicosis as like as natural occurrence.

The research work was conducted to evaluate the effects of stocking density on growth, survival and production of mirror carp (Cyprinus carpio var. specularis) spawn in nursery pond for a period of 35 days. Three treatments differing in stocking density of hatchling viz., T₁:172900 individuals/ha, T₂:148200 individuals/ha   and T₃:123500 individuals/ha were employed each having three replicates. Fries were fed same diet in three different treatments consisting of (28.5%) at the rate of 6-10% of body weight. The Physico-chemical characteristics of pond water were measured weekly. The mean values of some water quality parameters such as temperature (°C) were 30.41 ±1.15 (T₁), 30.38±1.08 (T₂), 30.45±1.10 (T₃); transparency (cm) 40.00±0.90 (T₁), 39.74±0.51 (T₂), 39.93±0.70 (T₃); dissolved oxygen (mg/l) 6.79±0.45 (T₁), 6.71±0.38 (T₂), 7.02±0.29 (T₃); pH 7.55±0.18 (T₁), 7.67±0.16 (T₂), 7.62±0.20 (T₃) and alkalinity (mg/l) 116.20±0.64 (T₁), 99.36±0.45 (T₂), 96.47±0.93 (T₃)  from 35 days respectively. Except alkalinity no significant value were found for parameters. Sampling was also done weekly. The mean value of final weight (g) was 0.35±0.004 (T₁), 0.42±0.008 (T₂), 0.59±0.006 (T_3­). The survival rate of Cyprinus carpio var. specularis was 54.20% (T₁), 62.90% (T₂) and 74.56% (T₃) respectively. The highest survivability was found in T₃. The production (kg/ha) of Cyprinus carpio var. specularis was 76.32±4.96 (T₁), 77.60±5.19 (T₂), 91.04±6.02 (T₃) respectively. Significantly (p< 0.05) highest production was found in T₃.

The study was conducted on Bergobindapur baor at Chaugachha upazila under Jessore district. The study period was February to June, 2015. Data were collected by using participatory rural appraisal (PRA) tools and personal observation. Net return was loss in 2013-2014 and the loss amount was BDT 254200. Cost benefit-ratio in 2013-2014 was 0.99. Major income (83.30%) of the fishermen community was drawn from the fishery activities of Bergobindapur baor. In 2014-2015, average income of fishermen was BDT 25250 during the fishing period. Within the household of fishermen community, the expenditure for food was 19%, clothing, 3%, children’s education, 19%, medical, 3%, dowry and religion, 33%, agriculture, 13% and others, such as business, accommodation, sanitation etc. 10%, which came from baor activities. The findings of this study indicated that Bergobindapur baor plays a vital role for the support of livelihood of fishermen and net profit from baor was not satisfactory. A long-term strategy should be developed to make the baor profitable. The developed strategic plans should be initiated immediately for the profitability of the baor as well as improving the livelihood conditions of the fishermen engaged with the boar fishery activities.

A field experiment was carried out at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh, during June 2013 to December 2013 to study the yield and grain dimensions of transplant aman rice varieties as influenced by date of transplanting. The experimental factors comprised of two factors namely, date of transplanting and variety. Date of transplanting comprised of 18 July, 19 August and 18 September. The variety comprised of Binadhan-7, BRRI dhan57, BR11 and Bashiraj. The experimental field was laid out in a split plot design. Most of the yield parameters were significantly affected by the date of transplanting. The yield and yield contributing characters were highest at 19 August transplanting and lowest at 18 September transplanting. Variety had significant effect on most of the yield and yield contributing characters. BR11 gave the highest grain yield (4.47 t ha^-1) and the lowest value was obtained in BRRI dhan57. Among the grain dimensions, Binadhan-7 with 18 July transplanting gave the highest grain length (9.64 mm) whereas BR11 with 18 September transplanting gave the lowest value (7.82mm). Results showed that yield components were highest at 19 August transplanting and after that the reduction occurred at 18 September may be due to low temperature and short day length at flowering stage.

Growth performance of guppy fries was assessed by providing four different types of formulated diets comprised of some available ingredients (fish meal (FM), prawn meal (PM), meat and bone meal (MBM), wheat flour (WF), corn flour (CF), starch (S) and soya bean oil) and the result was compared with supplied commercial diet as control. After 30 days experimental period, diet 1 (FM: PM=6:1) showed the best result in case of length gain (0.48 cm) in guppies whereas lowest (0.01 cm) was reported from diet 2 (PM: MBM= 4:3). The body weight gain in guppies was also found higher (0.004 g) in diet 1 than other diets. Specific growth rate (SGR) of all formulated diets was between 1.03% to 4.8% while 0.7% was found in commercial diet. Maximum survival rate (95%) was recorded in formulated diet 1 whereas 80% was investigated in control .Growth trend of guppies from all the supplied formulated diets resulted better rather than the imported commercial diets. So, Commercial diets might be replaced by experimental diets for the better production of guppy.

A study way carried out in Dhaka city to find out the management and treatment patterns of diabetic patients those having blood pressure complications. It was a cross sectional study of 150 patients by using self administered survey questionnaire and founded data was analyzed and described. In this study, it is found that almost all of the patients (92%) were from Dhaka and just were a few those were outsider but came as referred patients. The male and female percentages were accordingly 64.67% and 35.33% and among those diabetes patients 47.33% had hypertension and 10.66% had hypotension and most of them (74%) check their diabetes monthly. Among those patients 79.33% patients were prescribed only drugs while 17% patients were prescribed to change their routine only and 4% were prescribed both (drug and routine). Most of the diabetes patients having blood pressure complicacy were prescribed only drugs which is very significant and those are going to do further research in this aspect will be benefited by getting this information.

Investigation on the health condition of small indigenous fish, Channa punctatus was carried out through clinical and histological observation from Jailka beel of Sherpur sadar, Sherpur district and Kailla beel of Ishargonj upazila, Mymensingh district for a period of six months from October 2014 to March 2015. Water quality parameters like water temperature, pH, dissolved oxygen, ammonia, total hardness, alkalinity and nitrate were recorded. Water temperature and total hardness were found at unfavorable level for fish in December and January. Clinical examinations of the fish were also carried out for any kind of abnormalities at monthly intervals. Clinically, it was observed that fish was affected with numerous red spots and patches in lateral and ventral regions, large deep whitish ulcers reached up to deep ulcers especially in December and January in both the beels. Samples of skin, muscle, gill, liver and kidney were collected and processed for histological observations. Major pathology in the skin and muscle were epidermis separated from dermis, presence of fungal granuloma, vacuums, hemorrhage and necrosis. Loss of primary and secondary gill lamellae, hypertrophy and primary gill lamellae separated, necrosis and hemorrhage were found in the gill. Large vacuums, necrosis and hemorrhage were observed in liver and kidney. Among the affected fish organs, skin and muscle, gills were found to be more affected than those of the internal organs like liver and kidney. Overall, under the clinical and histopathological observations the fish were found to be more affected in December and January. Whereas, in the months of February and March, the pathological condition of fish gradually healed up to normal except few vacuums and hemorrhage. Under histopathological observations, fish of Jailka beel were more affected than the fish of Kailla beel. In clinical and histopathological observation C. punctatus was more affected due to EUS.

Stocking density is considered one of the important factors affecting fish growth. The study was aimed to compare the growth parameters of monosex tilapia at various stocking densities. The experiment was carried out during the period from 06 August to 22 December 2014. Three stocking densities were used as 125, 250 and 375 fish/dec and designated as treatment T₁, T₂ and T₃ respectively each having two replicates. All the fishes were of same age group having average initial body weight of 1.34g. A commercial feed was supplied at the rate of 40% of the body weight and then gradually it was readjusted to 20%, 10%, 5% and 3% respectively and continued up to the end of the experiment. The water quality parameters were monitored at 14 days interval and the ranges were: temperature 19.34 to 31.40°C, pH 6.83 to 8.03, dissolved oxygen 4.78 to 6.82 mg/l and transparency 29.02 to 49.45cm.The result of the present study showed that the mean weight gain was significantly (P<0.01) highest in T₁(120.58g) followed by T₂(89.74g) and T₃(74.58g).The average specific growth rates (SGR) was 2.590, 2.560 and 2.598 (%/day) in treatment T₁, T₂ and T₃ respectively. There was significant (P<0.01) differences among the survival rate. The survival rate 87% was significantly highest in T₁followed by 76% in T₂ and 69% in T₃.The fish productions were 13.25, 17.30 and 19.64 kg/decimal in T₁, T₂ and T₃ respectively. Although the highest production was obtained in T₃ but individually growth performance of monosex tilapia was highest in T₁. The highest net profit was found (BDT 3,373.30) inT₁compared toT₂ (BDT 3,017) and T₃ (BDT 2,918). The highest benefit cost ratio (BCR) was 1.79 in T₁followed by 1.44 in T₂and 1.28 in T₃. Based on the result of present experiment, fish farmers might be suggested to rear tilapia at lower stocking density to get higher growth, survival and benefit in a short period of time.

A nucleus-breeding flock of selected Brown Bengal goat was established in the Bangladesh Livestock Research Institute (BLRI), Regional Station, Naikhongchari, Bandarban, with the objective of characterization, conservation and improvement of the breed. A total of 69 does of different generations (Foundation = 07, Generation one = 32, Generation two = 23 and Generation three = 07.) and 07 bucks (All were first generation) were used to study the genetic parameters of Brown Bengal goat on productive and reproductive traits. The studied Animals were bred naturally. Goats were reared under semi-intensive management system in which goats were browsing in nature and allowed to graze in field as well as mounting in hills. All goats were housed in a plastic made floor house and allowed to graze 6-8 hours in a day and concentrate was offered twice daily during morning and evening at the rate of 1% of their body weight per day. The adult body weight of hilly goat was 20.95 kg. The phenotypic characteristics like the face, horn, ear and udder length were 15.56, 7.18, 12.19 and 12.67 cm respectively. On the other hand the hearth girth, front leg, hind leg and body length were 61.51, 46.8, 49.48 and 55.24 cm respectively. The gestation length, kidding to first heat, kidding to conception and kidding interval were 148.52 ± 1.06, 33.48 ± 2.85, 33.40 ± 1.98 and 176.86 ± 1.98 days, respectively. According to parity the GL, AFH, KC and KI were not followed trends up to 3rd parity but kid birth weight was increasing trends with increasing parity up to fifth. The birth weight of male kid (1.25 ± 0.25 kg) was higher than that of female kid (1.13± 0.27 kg). The highest birth weight (1.24±0.02 kg) was found in single birth. According to litter size the birth weight of single kid (1.24 ± 0.01 kg) stood first followed by twine (1.20 ± 0.01), triplet (1.1±0.03 kg) and quadruplet kids (0.93±0.08 kg).  . The productive and reproductive performances of brown Bengal goat were not affected by parity. Birth weights of kids were significantly varied with birth type.

 Reference ranges (RR) or Reference Intervals (RIs) are very important values for accurate interpretation of clinical laboratory test results. Any test result that is not accompanied by a valid RR value is less informative and may not be interpreted correctly. However, reference values of most analytes for Nigerian population are not readily available. The present study aims at establishing reference values of Serum Leptin and C-Peptide for Dutse, Jigawa State. In a cross sectional study, eighty (80) reference individuals were recruited. Serum Leptin and C-Peptide were analysed using ELISA methods. Following an accepted guideline, population specific reference intervals were established for these analytes and found to be 3.13ng/mL to 14.09ng/mL for Leptin and 0.56ng/mL to 5.64ng/mL for C-Peptide, respectively. Populations sharing similar physical and socio-economic characteristics may adapt these intervals if validated and considered suitable for their laboratory methods.