2017

This study was carried out to investigate the drug resistance pattern of Salmonella spp. from diarrheal patients of under 5 years of age. Collected stool samples from the hospitalized diarrheal patients were analyzed for the presence of Salmonella spp. Among 350 stool samples from diarrheal patients, 15 (4%) were positive for the Salmonella spp. Antibiotic susceptibility test of the identified Salmonella spp. was performed according to Kirby-Bauer disc diffusion method. Eight commonly used antibiotics including azithromycin (15 µg), chloramphenicol (30 µg), Sulphamethaxole-trimethoprim (25 µg), metronidazole (50 µg), tetracycline (30 µg), doxycycline (30 µg), erythromycin (15 µg) and ciprofloxacin (5 µg) used to determine drug resistance pattern of the identified Salmonella spp. Majority of the isolates were multidrug resistant showed resistance against more than three drugs. 73% of Salmonella spp. was resistant to 2-4 drugs and 20% showed resistance to more than 5 drugs. We found that 100% Salmonella showed resistance to metronidazole and 87% were resistant to erythromycin. 7% isolates were resistant to chloramphenicol and doxycycline and 20% were resistant to ciprofloxacin. Antibiotic resistance is a serious public health problem worldwide and a leading cause of mortality and morbidity. This kind of situation, leads to great socioeconomic losses from the perspective of the patient, the hospital, and the whole society. To get rid form the drug resistance problem unnecessary, over and misuse of antibiotics should immediately prohibited and policy of judicious use of antibiotics should be strengthened.

This epidemiological study was conducted to find out the incidence of Peste des Petits Ruminants (PPR) in goat and sheep at Upazilla Veterinary Hospital, Rangpur sadar, Rangpur during the period of January to April, 2014. In this period, 236 clinically infected goat and sheep were examined in which 22 (9.32%) PPR cases were diagnosed on the basis of history, clinical signs and gross pathological lesions. High fever (104-107 ^oC), mucopurulent oculo-nasal discharge, rapid and labored breathing, mouth lesion and diarrhea were the common clinical sign of PPR infected goat and sheep. The postmortem examination findings were dark red areas and congestion in different lobes of lungs, enlargement of spleen and lymph nodes, erosion of abomasums and characteristics zebra striping in the mucosa of colon. This present study reveals that about 7-12 months aged group of goats were more prone (40.91%) to PPR compare to adult (above 1 year) and Black Bengal goat was more susceptible (72.32%) than Jamunapari (27.78%) goat where the occurrence of  PPR disease was more in goat (81.82%) than sheep (18.18%).

An experiment was conducted in the poultry rearing unit of Sahjalal Animal Nutrition Field Laboratory, Bangladesh Agricultural University, Mymensingh for a period of 30 days by using  108 one week aged straight run Starbro broiler chicks to find out the effect of vitamin E and selenium as feed supplement for the performance of broiler. Chicks were distributed into 4 dietary treatment groups each having three replications (R1, R₂, R₃) and each replication contains 9 chicks. Dietary treatment groups were T₀ (Control i.e. without supplemental vitamin E and Selenium), T₁ (Control + 50 mg vitamin E 0.11 mg selenium per kg diet), T₂ (Control + 100 mg vitamin and 0.22 mg Selenium per kg diet and T₃ (Control + 150 mg vitamin E and 0.33 mg Selenium per kg diet). At end of the feeding trail the body weight of birds  were 1238.28, 1231.85, 1272.59, and 1218.33 gm/bird in treatment groups T₀, T₁, T₂ and T₃ respectively which differ significantly (p<0.05) where highest body weight observed in T₂ group . Body weight gain was highest in T₂ (control+100mg vitamin E and 0.22 mg Selenium per kg diet) and lowest in T₀ (control). Total Feed consumption during the experimental period was 2480.30, 2523.80, 2564.56 and 2555.39 g for T₀, T₁, T₂ and T₃ respectively. At 2^nd and 3^rd week of age, weekly feed conversion ratio were significantly (p<0.05) higher in T₂ group than the other groups including control. Meat yield parameter of broilers showed significant (P<0.05) differences in dressing weight, breast weight and shank weight. Based on the above findings, the result suggest that supplementation of  Vitamin E 100 mg and 0.22 mg Selenium per kg diet from ‘‘Lolyvit-ES’’ as a source of  Vitamin E and Selenium may be used in broiler rations.

The 9-cis retinoic acid (9-cisRA) enhances early embryonic development in both in vitro and in vivo conditions. This experiment was conducted to evaluate the effect of supplementation of 9-cisRA in the in vitro maturation (IVM) medium on embryo development efficiency and embryo quality. For this purpose, immature cumulus oocyte complexes (COC) collected from slaughterhouse derived bovine ovaries were matured in three different IVM media (control group, DMSO group and DMSO+RA group). In the control group, base IVM medium were used without supplementation of 9-cisRA and DMSO. In the DMSO group, base IVM medium was supplemented with 0.5 µl DMSO per ml IVM medium without 9-cisRA. In DMSO+RA group, base medium was supplemented with 5 nm 9-cisRA dissolved in 0.5 µl DMSO. Data were analyzed using one way ANOVA method and means were compared using Duncan’s multiple range test. Results showed that, supplementation of 9-cisRA in the maturation medium has no effect on embryonic development uptocleavage stage. However, blastocyst development rates (P>0.01), total blastomere number (P> 0.01), number of apoptotic blastomere per blastocyst (P>0.05) and percent of apoptotic blastomere per blastocyst (P>0.05) were significantly influences by 9-cisRA. In conclusion, 9-cisRA may be supplemented into the maturation medium for increasing bovinein vitro blastocyst development efficiency and blastocyst quality.

The  present  study  was  conducted  to  investigate  effects  of  stocking  density  on  growth  performances  of  gold  fish  (Carassius auratus)  in  hapas.  Experiment  was  conducted  for  a  month  with  three  treatments  where  three  stocking  densities  were  T₁  (10  fry/hapa),  T₂ (15  fry/hapa) andT₃ (20  fry/hapa)  each  having  three  replications  which  were  selected  randomly.  In  the  present  experiment  hapa (3ft × 2ft × 2ft)   with  1  mm  mesh  net  was  used.  Gold fish  fry  having  a  mean  body  weight  of  0.007 g  were  used  in  all  treatments. Fishes were fed at the rate of 10% of their body weight containing 34.11% protein. Water quality  parameters were monitored at 10 days interval and the ranges were –temperature 24.75 to  27.75 ^oC, dissolved oxygen 3.68 to 4.09 mg/L, p^H  7.3 to 8.16, ammonia 0.3  to  1  mg/L,  nitrite  0.01  to  0.03  mg/L,  phosphate  0.6  to  1  mg/L  and  alkalinity  119  to  187 mg/L.  At  the  growth  performances  were  evaluated  by  comparing  mean  final  body  weight,  specific  growth  rate  and  food  conversion  ratio.  The  present  study  showed  that  the  gold  fish  fry  in  T₁  resulted  the  best  mean final  weight  gain  (1.188 g)  followed  by  T₂  (0.834 g)  and  T₃ (0.686 g).  The  SGR  ranged  between  6.64  and  7.43%  per  day  and  FCR  ranged  between  3.56  and  4.12  with  T₁  showing  the  lowest  FCR.  The survival rate (%) ranged between 76.67% to 85.67%.  From  the  present  experiment  it  was  found  that individual  fish  growth  rate  was  decreased  with  the  increase  of  stocking  density.

This study was planned to clarify the variation of body weight and average daily gain traits in both indigenous and F1 crossbreed (Local×Mediterranean) buffalo at weaning (sixth month) and yearling stages (twelfth month). Data were collected from 33 indigenous and 35 F1 crossbred buffalo progeny during the period of 2011 to 2014 with intensive management system at Research and Development farm of Lal Teer Livestock Limited in Bangladesh. The gathered data were analyzed using general linear model (GLM) and independent sample t test. The effect of parity, sex, season and breeding year on body weight and average daily gain were observed where significant values found in breeding period (p <0.01) of crossbred buffalo for body weight. It might be the reason of crossbreeding to indigenous buffalo cows using imported semen with better genetic merit. The parity (p <0.05), season (p <0.05) and breeding period (p <0.01) were also significant for average daily gain measurement which suggesting environmental adaptability beside additive genetic effects. Body weights were measured at weaning and yearling stages for both local (113 to 195 kg) and F1 crossbred (144 to 220 kg) buffalo progeny to screen the elite offspring for the Genetic Farm and Bull station. Similarly, average daily gain observed 469 to 457 g and 600 to 506 g at the previously mentioned two stages for both local and F1 buffalos, respectively. It was observed that studied traits were comparatively better in F1 buffalo progeny than indigenous. The comparison of average daily weight gains between indigenous and F1 buffalos at weaning and yearling periods showed significant differences for body weight (p <0.001) and average daily gain (p <0.05), respectively. It is concluded that genetic gain might be possible in local buffalo population by grading up process which could switch the traditional buffalo rearing to commercial level as well as meeting the nutritional demand of country people.

Cypermethrin, the synthetic pyrethroid commonly used as a pesticide, contaminates the aquatic ecosystem as a toxic pollutant from agricultural and domestic washouts. An experiment was conducted to carry out an empirical study to investigate the sub-lethal effects of LC₅₀ value of a pyrethroid pesticide, cypermethrin 10EC on histological changes of kidney in Tengra, Mystus tengara at wet laboratory of the Faculty of Fisheries, Bangladesh Agricultural University (BAU), Mymensingh. The LC₅₀ value of cypermethrin 10EC was calculated by probit analysis and LC₅₀ value for 96 hours was found 0.133 ppm. The experiment was conducted with four treatments, each with three replications. Treatment one (T1) was used as control (0 ppm) and three concentrations, such as 0.026 ppm (20% of 96 h LC₅₀), 0.052 ppm (40% of 96 h LC₅₀) and 0.104 ppm (80% of 96 h LC₅₀) were used as Treatment two (T2), Treatment three (T3) and Treatment four (T4), respectively. For histological study kidney of studied fish were collected from control and experimental group at 7, 14 and 28 days interval up to the end of experiment of 28 days. The physical reactions observed in the treated fish were erratic swimming, discolorations of the skin, loss of reflex, hyperactivities, motionless state and these effects increased with increasing concentration of the toxicants and duration of exposure. The changes observed in the kidney tissues were vacuolation, necrosis, ruptured kidney tubules, Cellular degeneration and karyolysis were recorded. Cypermethrin 10 EC has adverse effects on the organs of fish, so it should not be used indiscriminately in agriculture and aquaculture.

Aqua feeds have been considered as a major subsector in aquaculture. About 25 commercial fish feed industries in Bangladesh are now formulating over 1 million tones of commercial pelleted feed for the use by aquaculture farmers. A survey of different fish feed industries, their produced feed, used raw materials, machineries, distribution and marketing channel was carried out in 2015 at Noakhali of Bangladesh. Four fish feed industries were found having legal permission to produce fish feed in Noakhali. These were Globe-agro vet Ltd, Partex agro fish feed Ltd, Setu feed mills and R.P (Rasulpur) fish feed Ltd with an area of 9, 3, 2 and 3.5 acre, respectively. They used different types of machineries. Maximum machineries were found in Globe-agro vet Ltd. They used 19 types of raw materials where some were local and some were imported. Maximum raw materials were found in Globe-agro vet Ltd and they used 16 types of raw materials. Four types of fish feed were being produced in four fish feed industries and these are nursery, starter, grower, finisher. Square company was common of different raw materials supplier. Feed companies were linked with other business and take loan from local NGO and foreign organization. Their production capacity was not same rate all round the year. They like to produce feed from March to October. There was not so far difference in proximate composition of surveyed feed industries. Protein ranged at 28-35%, Lipid 5-8 %, moisture 10-12 % and Ash 12-18 %. The government should move forward with financial as well as technical support for the expansion and modernization of fish feed industries with a view to protecting the nature from incursive pollutants generated during feed manufacturing process.

Effects of different chemotherapeutics were examined against experimentally infected stinging catfish Heteropneustes fossilis. Fish were collected from a fish market in Mymensingh, acclimatized for 7 days in laboratory condition from January to February, 2016 in aquaria at Fish Clinic of Bangladesh Agricultural University, Mymensingh, experimentally injured by using forceps and knife and waited seven days for the establishment of infection. Before starting chemotherapeutic trial, it was confirmed that the fish were infected with microorganisms by clinical diagnosis in laboratory condition. They showed hemorrhages and ulcerative lesions over the infected area. A total of 80 such experimentally infected stinging catfish having average body weight of 18 g were used for the experiment. Two chemotherapeutics: antibiotic, Eryvet (erythromycin thiocyanate INN, sulphadiazine (NaUSP) & trimithoprim BP) and antifungal, methylene blue were used in separate and combined treatment with three different doses of antibiotic. The same dose of methylene blue, 0.2 mg/l, was used for separate and combined treatment by antibiotic. Doses of antibiotic (Eryvet) were 0.8 g/10 kg body weight of fish, 1g/10 kg body weight of fish and 1.2 g/10 kg body weight of fish as lower dose, recommended dose and higher dose respectively. For combined treatment the above different doses of antibiotic and the same dose of antifungal were used. Water was exchanged regularly. The chemotherapeutic trial was conducted for 7 days and observation was continued for another 8 days to observe the effect of treatment. Combined treatment with the recommended dose of the antibiotic and methylene blue showed the best result where 90% fish were recovered. By the treatment with the higher dose of the antibiotic 70% fish were recovered. Antifungal treatment showed that 20% fish were recovered. All the fish in negative control aquarium died.

The present study was conducted to assess the livelihood status of fish farmers and socioeconomic condition in the Jhikargachha upazila, Jessore, Bangladesh. Data were collected from 50 fish farmers for a period of six months from June 2016 to November 2016. The study indicated that 38% of the farmers were in age structure of 50-60 years, 44 farmers (88%) were Muslims and others Hindu (12%). About 82% farmers had a primary occupation of agriculture and others were involved in business (8%), service (4%) and politics (4%). It was found that, 66% farmers had joint family and others lived in separated family (34%). Majority (44 farmers) respondents had concrete house and rest of (6 farmers) had semi-concrete house. Almost (44%) farmers used concrete toilet and rest of farmers used semi-concrete (56%). All the respondents used to drink tube well water for drinking and other household works. Out of 50 fish farmers, 4% had no education (illiterate), 36% had primary education, 42% secondary level (Up to X), 10% S.S.C., 4% H.S.C. and 4% bachelor level of education. In the study area, 32% of the farmers had ponds of 34-66 dec, 28% had pond of 15-33 dec, 14% had ponds of up to 100 dec, 22% had ponds of 101-330 dec and 4% had ponds of 330-above dec. It was found that, 62% farmers had training on fish farming and rest 38% farmers had no training on fish farming. Among 50 farmers 64% farmers were found used to invest their own credit and rest 36% farmers took loan from bank, NGO, money lender and broker agency. Most of the farmers were interested to stock rui, catla and mrigal and other species. In the study area, cow dung was used by 50 (100%) of farmers, 47 farmers used urea, 40 farmers used TSP and 34 farmers used MoP. It was found that, 40% farmers produced fish between 1001 kg – 2000 kg/year and 17 (34%) farmers produced 3001 kg-above/year. The highest income was 3,30,75,000Tk/Year and the lowest income from a pond was 20000 Tk/Year. In the study area, every farmer had at least one mobile phone. Almost 96% farmers were used to go MBBS doctor and only 4% to homeopathic treatment.

An experiment was conducted at the Soil Science Field Laboratory of Bangladesh Agricultural University, Mymensingh during boro season of 2016 to evaluate the effect of deep placement of nitrogen (N) fertilizers on N use efficiency and yield of BRRI dhan29 under continuous flooded condition.  The soil was silt loam in texture having pH 6.27, organic matter content 1.95%, total N 0.136%, available P 3.16 ppm, exchangeable K 0.095 me%, available S 10.5 ppm and EC 348 μS cm^-1. The experiment was laid out in a Randomized Complete Block Design (RCBD) with eight treatments and three replications. The treatments were T₁ [Control], T₂ [Prilled Urea, 130 kg N ha^-1] , T₃ [USG, 130 kg N ha^-1], T₄ [USG, 104 kg N ha^-1], T₅ [USG, 78 kg N ha^-1], T₆ [NPK briquette , 129 kg N ha^-1], T₇ [NPK briquette, 102 kg N ha^-1] and T₈ [NPK briquette, 78 kg N ha^-1]. All the treatments except T_6, T₇ and T₈ received 25 kg P and 64 kg K ha^-1 as TSP and MoP, respectively. In T_6, T₇ and T₈ treatments, P and K were supplied from NPK briquettes. Prilled urea was applied in three equal splits. USG and NPK briquettes were applied at 10 DAT and were placed at 8-10 cm depth between four hills at every alternate row. The results demonstrate that all the yield components except 1000-grain weight and yields of BRRI dhan29 responded significantly to the deep placement of N in the form of USG and NPK briquettes under continuous flooded condition. The highest grain yield of 6561 kg ha^-1 was recorded in T₃ [USG, 130 kg N ha^-1] which was statistically similar to that ofT₄ [USG, 104 kg N ha^-1].The highest straw yield of 6876 kg ha^-1 was obtained in T₃ [USG, 130 kg N ha^-1]. The lowest grain yield (3094 kg ha^-1) and straw yield (3364 kg ha^-1) were found for T₁ (Control). The deep placement of USG and NPK briquettes enhanced the recovery of applied N and N use efficiency in comparison with the broadcast application of PU. The highest value of NUE (32.05 kg grain increase per kg N applied) was obtained in T₅ [USG, 78 kg N ha^-1] followed by T₄ [30.75 kg grain increase per kg N applied) and the lowest value was found in T₈ [130 kg N ha^-1 from PU]. Based on yield, N use efficiency and cost-benefit analysis, an application of 104 kg N ha^-1  as USG can be recommended as the best treatment for achieving satisfactory yield of boro rice (cv. BRRI dhan29) at BAU farm and at adjacent areas under AEZ 9 (Old Brahmaputra Floodplain).

The study was conducted to investigate the status of cattle diseases with relation to seasonal variation in Dinajpur district of Bangladesh. A total of 11402 clinical cases were registered for the clinical diagnosis and therapeutic purposes at sadar upazila veterinary hospital in Dinajpur district of Bangladesh during the period of March, 2016 to February, 2017. Disease diagnosis was made on the basis of owner’s statement, general examination and clinical signs. Diagnosed diseases were categorized as general clinical disorders, parasitic diseases, viral diseases, bacterial diseases, venereal disease, and metabolic disorders. According to study the highest (64.15%) prevalence was by parasitic disease followed by bacterial diseases (10.67%), viral diseases (8.97%), metabolic disease (7.61%), and venereal disease (6.60%). In respect of seasons, the prevalence of both bacterial diseases and viral diseases were significantly (p<0.01) higher (13.88%) and (12.10%) in summer season respectively. Whereas the parasitic diseases were significantly (p<0.01) higher (68.65%) in winter season. On the basis of season, it was found that the overall prevalence of diseases was highest in summer season (47.87%) followed by rainy season (30.10%) and comparatively lower in winter season (22.03%).

The study was conducted to monitor the microbial assessment in oropharyngeal swab, cloacal swab and faecal samples of ostrich and also update knowledge on antimicrobial sensitivity of isolated organisms. The study was performed at Hajee Mohammad Danesh Science & Technology University (HSTU) ostrich farm from January to June, 2016. A total 75 samples were randomly collected from 8 ostriches at different age of which 25 oropharyngeal, 25 cloacal swabs sample and 25 were faeces samples. The organisms were isolated by using standard microbiological method. The result revealed that the average microbial load in plate count agar was highest in faeces (1.48×10⁹±0.27 CFU) than oropharyngeal swab (1.36×10⁹±0.38 CFU) and cloacal swab (1.28×10⁹±0.34 CFU). Among 75 samples, 29 (38.66%) Escherichia coli, 21 (28%) Salmonella spp., 20 (26.67%) Staphylococcus spp. and 5(6.6%) Bacillus spp. were identified. In 25 oropharyngeal swab samples, Staphylococcus spp. was found highest 15 (60%) than other organisms but Escherichia coli were found highest both in cloacal 11(44%) and faecal 12(48%) samples. On antibiogram study Escherichia coli, Salmonella spp., Staphylococcus spp. and Bacillus spp. were more sensitive to Levofloxacin, Azithromycin, Nalidixic Acid and Amoxicillin respectively. While more resistant to Azithromycin, Penicillin, Chloramphenicol and Erythromycin respectively. The obtained results indicated that ostrich excreta are one of the most important sources of Escherichia coli, Staphylococcus spp., Salmonella spp., Bacillus spp. in ostrich farms. Finally, it may be concluding that the logical use of antibiotics must be adopted in ostrich farms reared in Bangladesh for prevention of the appearance of multidrug resistance bacteria. Moreover, proper measures should be taken to aware off zoonotic diseases in peoples who are involved in ostrich farming.

Native chickens of Bangladesh are scavenging in nature. In our previous studies, we have histologically and immunohistochemically examined the ontogeny of lymphoid organs of native chickens of Bangladesh in prenatal stages. To date, the study on gross anatomical development of prenatal lymphoid organs is lacking. In the present study, detailed macro-anatomical development of prenatal lymphoid organs of native chickens were examined during different embryonic stages of development, which was conducted in the laboratory of the department of Anatomy and Histology, Bangladesh Agricultural University. In the present study, thymus was first identified by the 10 days of incubation (ED 10) as a chain at the both side of the neck. Bursa of Fabricius and spleen were also first found to develop at ED 10. While comparing with the prenatal development of lymphoid organs of native chickens with that of previously studied hybrid chickens, the native chickens’ lymphoid organs were found to develop earlier. Taken together with our previous results, the developmental trajectory data of the present study are first to clarify that the immune system of native chickens are being prepared earlier to face the scavenging environment after hatching.

Cardiovascular diseases (CVD) are the leading cause of morbidity and mortality not only in Bangladesh but also all over the world. In Bangladesh, Women are more vulnerable group in all socio economic indexes. A cross-sectional study was conducted among 300 women from Dhaka City. Samples were chosen purposively Data were collected by semi structured self administered questionnaire. Questionnaire was based on Likert scale. Data analysis was performed by using Statistical Package for Social Science - 17.0 for windows. 75.4% respondent were in the age group of 18-35 years (Mean 30.56, Min:18 years and Max: 68 years). Majority (89%) were married & Muslim (99%) by religion, half of the participant (49.3%) were completed only primary education which indicate the poor status of women education in Bangladesh. The family size of most of the respondent (58%) are 4-6. Very few respondent have large family. It was found that 56.7 % of respondents had good knowledge, 8.3 percent had satisfactory knowledge, 1.7% had excellent knowledge and the remaining 29 percent had poor knowledge regarding cardiovascular diseases. The study revealed that among the total respondent, 99.7% women showed negative attitude & told that they would never involved in smoking. On the other hand 27.7% women show negative attitude & told that the take non smoke tobacco. Among women 56.67% respondent attitude were Moderately Favorable followed by 29% respondents were Favorable followed. Remaining 14.33% respondent attitude were favorable attitude. This study revealed that good knowledge as well as positive attitude towards major risk factors of cardiovascular diseases among the women is unsatisfactory. Socio-demographic factors for good knowledge and good knowledge for the development of positive attitude play vital role. The problem of cardiovascular diseases cannot be solved by Government alone. For effective prevention and control of cardiovascular disease, Govt. & private organization need to make integrated initiative in order to minimize the problem. [/showhide]

The present study was carried for the isolation, identification of bacterial pathogens from cloacal swabs of turkeys during the period from January-June, 2016. The entire research work was conducted in the Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur. The study was performed with 48 cloacal swab samples. The cloacal swab samples were collected carefully from three different Turkey Farms randomly and transferred aseptically to the laboratory. On the basis of morphology, staining, cultural and biochemical characteristics it was found that among the isolates 25(52.08%) samples were positive E. coli, 10(20.83%) samples were positive for Salmonella spp., 9(18.76%) samples were positive for both E. coli and Salmonella spp. and 4(8.33%) samples shown no growth in subculture media. Antibiogram profiles indicate that E. coli isolated were 100% sensitive to Azithromycin, Kanamycin and Ciprofloxacin, 80% sensitive to Cefradine, Vancomycin and Levofloxacin, 60% sensitive to Cefotetan and Nitrofurantoin and 40% sensitive to Erythromycin. The isolates were 100% resistant to Cloxacillin and Cefixime. On the other hand, Salmonella spp. were 100% sensitive to Azithromycin, Kanamycin, Levofloxacin and Ciprofloxacin, 80% sensitive to Nitrofurantoin and Teicoplanin, 60% sensitive to Vancomycin, Erythromycin and Cefixime and 20% sensitive to Cefotetan. The isolates were 100% resistant to cefradine and cloxacillin. So, for E. coli Azithromycin, Kanamycin and Ciprofloxacin were more sensitive and for Salmonella spp. Azithromycin, Kanamycin, Ciprofloxacin and Levofloxacin were highly sensitive. Diversified bacterial species were present in cloacal swabs of Turkeys. However, E. coli, Salmonella spp. infection might make the birds vulnerable for easy access of infection. It could be concluded that E. coli and Salmonella spp. may pass through the feces to the environment. It causes a potential human health hazards and can cause illness.

This clinical study was undertaken at the sadar Upazilla Livestock Office, Gopalgonj, Bangladesh to determine the general clinical prevalence of clinical diseases and disorders in cattle and goats during the study period from September to December 2016. Total animals were 105, among them cattle were 60 and goats were 45. Diagnosis was performed on the basis of general examination, physical examination and clinical examination. Clinical examinations were detected 60 (48.38% of total animals) different types of diseases and disorders in cattle. Whereas fever 3, anorexia 5, bloat 2, dysentery 3, diarrhoea 4, fascioliasis 2, myiasis 5, FMD 2, papillomatosis 2, ephemeral fever 3, pneumonia 5, mastitis 4, black quarter 1 were recorded. Age wise occurrence in young and adult cattle were 35 (58.33%) & 25 (41.66%) respectively. In goat 45 (36.29% of total animal) different types of diseases and disorders were found. Whereas fever 4, anorexia 4, bloat 1, dysentery 2, diarrhoea 3, corneal opacity 2, mange 3, myiasis 4, PPR 10, pneumonia 8, tetanus 1, mastitis 3 were recorded. Age wise occurrence in young and adult goat were 20 (44.44%) and 15 (33.33%) respectively. Diseases and disorders of cattle and goats which were recorded more or less present in both young and adult animals but some of the specific diseases and disorders were in animals due to age susceptibility such as black quarter in young cattle and PPR in goat. From this study it can be said that proper vaccination, treatment and management should be given to minimize the diseases and disorders of cattle and goat.

Antibacterial active compounds isolated from higher plants appear to be one of the most important alternative approaches to contain antibiotic resistance and the management of disease. The present study was aimed at to investigate the antibacterial activity of some medicinal plant species including seeds namely Centella asiatica, Oxalis corniculata, Phoenix dactylifera, Clitoria ternatea and Nigella sativa. The methanolic and ethanolic extract of Centella asiatica, Oxalis corniculata, Phoenix dactylifera and Clitoria ternatea was investigated for antimicrobial activity at different concentrations by using disc diffusion method against some bacterial strains with standard antibiotic cephachlore (10 μg). The result demonstrated that all the extracts showed antibacterial activities against the isolates, which were dose dependent. The ethanolic extract of Oxalis corniculata at a concentration of 50 μl/disc had the highest effect on the test organism B. subtilis, Salmonella typhi and E. coli with 9.3, 9.7 and 10.8 mm diameter of zone of inhibition respectively while at the same concentration the extract of Clitoria ternatea had a higher antimicrobial activity on Bacillus cereus with 11.3 mm diameter of zone of inhibition. The activities of the plant extracts on the tested pathogens were not as effective as the standard commercial antibacterial disc but was significant. In case of Nigella sativa, Pseudomonas aeruginosa was sensitive to both the ethanolic extract and oils at different concentration. Salmonella typhi shows the same degree of inhibition for ethanolic extract as Bacillus cereus, but greater inhibition for oils. Of all the bacteria, Pseudomonas aeruginosa was the most sensitive bacteria against ethanolic extract and oils, while Bacillus cereus was the most resistant bacteria. Generally, the oils of Nigella sativa sample had higher antibacterial activity against bacteria than ethanolic extract. All the extracts are inhibitory to most common human pathogen S. typhi. The results suggest that the different plant extracts has a significant scope to develop a novel broad spectrum of antibacterial herbal formulations.